Determination of the high molecular weight impurities in cefsulodin sodium by Sephadex G-10 gel chromatography SephadexG-10凝胶色谱条件分析头孢磺啶钠的高分子杂质
Methods The EtOH extract of radixes of Daphne genkwa was initially separated with silica gel chromatography eluted by petrol/ acetone gradient. 方法芫花根醇提物以硅胶柱层析,石油醚/丙酮梯度洗脱。
The determination of molecular weight distribution of PVC by gel chromatography and its application in quality control 采用凝胶色谱法测定PVC分子质量分布及其在质量管理中的应用
Some fractions with low molecular weight have been obtained by silica gel chromatography separation of Pingshuo coal extracts. 用硅胶色谱柱将平朔烟煤吡啶抽提物分离成八种不同馏分。
The cultural fruiting bodies of the Gymnopilus spectabilis were phytochemical investigated using layer extracts with petroleum ether, diethyl ether, methanol and water and silica gel chromatography. 本研究采用石油醚、乙醚、甲醇、水溶剂梯度提取法和多种柱层析法,对栽培橘黄裸伞子实体进行了化学研究。
Methods: Crude polysaccharide was extracted from Russula fruit bodies through soaking extraction method in hot water and purified through ion-exchange chromatography and gel chromatography. 方法:用热水浸提法从红菇子实体提取粗多糖,并进行离子交换层析及凝胶层析纯化。
This experiment aimed to study the relationship between DH and MW of the hydrolysates of egg white by gel chromatography. 本实验应用凝胶色谱法研究了蛋清蛋白水解物的水解程度与分子量的关系。
It introduced the gel chromatography to determine the average molecular weight and molecular weight distribution of high polymer. 介绍了用凝胶色谱法测定高聚物的平均分子量及分子量分布。
ConclusionThe co-utilization of silica gel column and gel chromatography can greatly improve the efficiency of isolation and purification. 结论硅胶柱色谱和凝胶柱色谱的混合利用可以提高供试品的分离纯化效果。
Application of gel chromatography in development of matt PVC resin 凝胶色谱法在消光PVC树脂研制中的应用
MethodsThe chemical constituents were isolated and purified with silica gel chromatography, their structures were determined by spectral analysis and chemical evidence. 方法利用各种色谱技术进行分离纯化,根据化合物的理化性质和光谱数据进行结构鉴定。
Metachromasia, electrophoresis, and gel chromatography have shown that the charge of MPS and its chain length remain unchanged. 利用药物的变色反应性,电泳和凝胶色谱法显示MPS(多磺酸粘多糖)的电荷及其链长没有改变。
Determined by silica gel chromatography and high-performance liquid chromatography, the bioconversion ratio of hydrocortisone was34%. 经硅胶层析和高交液相色谱测定,氢化可的松转化率为34%。
At first, dSE was extracted, isolated and purified by ion-exchange resin and silica gel chromatography for preparing the qualified material for metabolism research. 首先利用离子交换树脂法及硅胶柱层析技术自一叶萩叶中,提取、纯化得到右旋一叶萩碱(dSE),为代谢研究准备了合格药物;
BH-1 was isolated and purified from the fermentation broth by rough fractionation and Sephadex G-100 gel chromatography. 将发酵上清液经粗分级分离和SephadexG-100凝胶过滤,得到具有纤溶活性的柱层析产物BH-1。
Comparison of the Determination of Polymer in Human Albumen Preparation by HPLC with Gel Chromatography 高效液相色谱法与凝胶层析法检测人血白蛋白制剂中多聚体的比较
Methods: To determine the content of polysaccharides by spectrophotography and analyse by gel chromatography. 方法:分光光度法测定多糖含量和凝胶色谱法分析。
Method: RRTP-1 was obtained by ion exchange column chromatography and gel chromatography. 方法:采用离子交换柱层析和凝胶柱层析纯化得到RRTP;
The polysaccharide gave a single peak on gel chromatography with Sephadex G-200 and high voltage electrophoresis. 该多糖经SephadexG&200凝胶层析柱和高压电泳分析证明是单一成份。
Method: Determination of expansion degree and analysis by gel chromatography. 方法:膨胀度测定法和凝胶色谱法。
Oleuropein was also separated from the secondary metabolite of the callus culture via silica gel chromatography and prep. 通过硅胶柱层析并结合薄层层析和制备高压液相,从培养得到的愈伤组织中分离鉴定出目标次生代谢产物橄榄苦甙。
Methods: We applied ether extraction and gel chromatography to separate anthracene derivatives from aloe. 方法:用乙醚萃取,弃水相,蒸干乙醚,凝胶层析柱分离得蒽衍生物。
The bioactive substances were purified through adsorption chromatography, gel chromatography and so on. The purity tests of HG-1 and HG-2 by TLC, analytical HPLC and chemical methods proved that they were both single substance. 用吸附色谱法、凝胶过滤色谱法等方法对该活性成分进行分离和纯化,并用高效液相色谱法、薄层色谱法和化学方法对其纯度进行检验,得到2个纯物质HG1和HG2。
Therefore, phenol extraction and gel chromatography can get the higher purity lipoteichoic acid. 因此,进一步证明冷酚法提取并通过凝胶色谱层析能够得到纯度较高的脂磷壁酸。
The purification technology and parameters for 32 kDa were researched with ion exchange chromatography and gel chromatography, detected with SDS-PAGE. 采用离子交换层析、凝胶层析,以SDS-PAGE检测,对其中一个蛋白进行了分离纯化研究,得到了32kDa蛋白的纯化工艺和参数。
Four antimycin components were isolated and purified from the fermentation broth by the combination of silica gel chromatography and high speed countercurrent chromatography ( HSCCC) methods. 此外实验建立了硅胶层析与高速逆流色谱相结合的现代分离纯化方法,对抗霉素的发酵液进行了分离纯化,并成功的从中分离出四个抗霉素的单一组分。
The average molecular weight in polysaccharides was analyzed by gel chromatography ( GPC). 在多糖平均分子量的研究中,使用凝胶色谱(GPC)进行分析。